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AI Startups Rewrite Venture Capital Playbook in 2025
14 Nov
Summary
- AI startups leaping from "zero to $100 million in revenue in a single year"
- Investors focus on data generation, competitive moat, founder experience, and technical depth
- Rapid growth no longer guarantees follow-on funding for early-stage AI companies

As of November 14th, 2025, the venture capital (VC) landscape has undergone a significant shift when it comes to backing AI startups. According to Aileen Lee, founder and managing partner of Cowboy Ventures, the rules of investing have changed dramatically, with some AI companies now leaping from "zero to $100 million in revenue in a single year."
However, this rapid growth is no longer the sole focus for Series A investors. Lee explains that they are now applying an "algorithmic formula" that considers factors such as the startup's data generation, competitive moat, founder accomplishments, and technical depth of the product. "Depending on what your company is, the output of the algorithmic formula is going to be different," she says.




